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Chem Commun (Camb) ; 57(92): 12270-12272, 2021 Nov 19.
Article in English | MEDLINE | ID: covidwho-1506302

ABSTRACT

An automated Cas12a-microfluidic system was constructed to distinguish the B.1.617.2 (delta) variant of SARS-CoV-2 from the wild-type virus rapidly and was validated using 30 clinical samples, showing 100% consistency with next-generation sequencing. It will be a potential tool for the rapid differential diagnosis of the delta variant of SARS-CoV-2.


Subject(s)
COVID-19/diagnosis , CRISPR-Cas Systems/genetics , Microfluidics/methods , SARS-CoV-2/genetics , Automation , COVID-19/virology , High-Throughput Nucleotide Sequencing , Humans , Mutation , Polymerase Chain Reaction , RNA, Guide, Kinetoplastida/genetics , RNA, Guide, Kinetoplastida/metabolism , RNA, Viral/metabolism , SARS-CoV-2/isolation & purification
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